Autoregulation of phosphorylation of the nicotinic acetylcholine receptor
نویسندگان
چکیده
منابع مشابه
Autoregulation of phosphorylation of the nicotinic acetylcholine receptor.
We have investigated the regulation of phosphorylation of the nicotinic ACh receptor (nAChR) in rat myotubes by the agonist carbamylcholine. Treatment of primary rat myotube cultures with carbamylcholine resulted in a 100% increase in phosphorylation of the nAChR gamma- (52 kDa) subunit and a 30% increase in phosphorylation of the nAChR delta- (62 kDa) and delta'- (66 kDa) subunits. These respo...
متن کاملTyrosine phosphorylation of nicotinic acetylcholine receptor mediates Grb2 binding.
Tyrosine phosphorylation of the nicotinic acetylcholine receptor (AChR) is associated with an altered rate of receptor desensitization and also may play a role in agrin-induced receptor clustering. We have demonstrated a previously unsuspected interaction between Torpedo AChR and the adaptor protein Grb2. The binding is mediated by the Src homology 2 (SH2) domain of Grb2 and the tyrosine-phosph...
متن کاملConformation of acetylcholine bound to the nicotinic acetylcholine receptor.
We report here the biologically active conformation of acetylcholine when bound to the high-affinity state of the receptor from Torpedo californica. The acetylcholine conformation was determined in the free and bound states by proton NMR two-dimensional nuclear Overhauser effects. In agreement with x-ray crystallographic data, acetylcholine in solution has an extended conformation with an avera...
متن کاملDetermination of the sites of cAMP-dependent phosphorylation on the nicotinic acetylcholine receptor.
The nicotinic acetylcholine receptor is a substrate for cAMP-dependent protein kinase both in vitro and in vivo. Recently, it has been demonstrated that phosphorylation of the nicotinic receptor by this kinase increases its rate of rapid desensitization. We now report the identification of the cAMP-dependent phosphorylation sites on the gamma and delta subunits. Two-dimensional phosphopeptide m...
متن کاملComparison of innervation and agrin-induced tyrosine phosphorylation of the nicotinic acetylcholine receptor.
Studies of the regulation of tyrosine phosphorylation at the neuromuscular junction during development and following denervation suggest that tyrosine phosphorylation of the nicotinic acetylcholine receptor is regulated by neuronal innervation of muscle. The finding that agrin, a neuronally derived extracellular matrix protein also induces tyrosine phosphorylation of the nicotinic receptor, sug...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1994
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.14-05-03271.1994